Fig. 4: Vermal PCs bidirectionally modulate CS firing via the FNE–IO pathway and shape medial cerebellar outputs.
From: Excitatory nucleo-olivary pathway shapes cerebellar outputs for motor control
a, Schematic illustrating vermal PC recording while photoinhibiting PCs in the same region. b, Left: example PC recording and raster plot of SSs and CSs following 10-ms photoinhibition. Right: population summary of the SS (turquoise) and CS (black) responses (n = 31 PCs from 4 mice). Activity traces are plotted as mean ± s.e.m. c, Example raster plots of CS activity following 10-ms, 250-ms and 500-ms PC photoinhibition. d, Population summary of the spike rates and peak timings of evoked CSs (n = 31, 37 and 32 PCs for each condition from 4 mice). Left: PSTH traces are plotted as mean ± s.e.m.; right, dots represent individual cells and bars represent mean ± s.e.m. (one-way ANOVA with Tukey’s multiple comparisons, P = 0.32). e–h, Same as (a–d), but for vermal PC recording while photoactivating PCs in the same region. Color codes in (g) and (h) indicate different photoactivation durations as those in (c) and (d), n = 20, 25 and 21 PCs for each condition from 3 mice. i, Left: average PSTHs (mean ± s.e.m.) of FN neuron activity (n = 40) and CS activity (n = 42 PCs) following 10-ms photoactivation of the FNE–IO neurons. See experimental strategies for FN recording in Extended Data Fig. 6, and for PC recording in Fig. 3. Right: distribution histogram of CS peak timing (black bars) and FN trough timing (red bars). j, Schematic showing recording and activation of FNE–IO neurons before and after pharmacologically inhibiting mcMAO. k, Raster plots and PSTHs of an example FN neuron before (black) and after (blue) muscimol infusion in the mcMAO. Red arrowhead indicates the putative CS-mediated feedback inhibition, which is abolished after mcMAO inhibition. l, Group average FN neuron activity (mean ± s.e.m.) and comparison of the putative CS-mediated feedback inhibition before and after muscimol infusion (n = 15 from 6 mice, two-sided paired t-test, P = 1.47 × 10−6). Dots represent individual cells and bars represent mean ± s.e.m.
