Extended Data Fig. 1: Distribution of FN axons in the cMAO.
From: Excitatory nucleo-olivary pathway shapes cerebellar outputs for motor control
Related to (Fig. 1a–d). a, Bulk injection of anterograde AAV9-tdTomato unilaterally in the FN (1/3 mice displayed). b, Representative images along the rostral-caudal axis, and quantification of FN axons in four subnuclei of the cMAO (n = 3 mice, mean ± s.e.m.). The projection density in each subnucleus is normalized to the total axonal labeling in the cMAO. c, Summary of the excitatory and inhibitory FN projections in the cMAO (related to the experiments in Fig. 1a,b). Top: superimposed cMAO images from VGluT2-Cre (magenta, 1/4 mice displayed) and Gad2-Cre (cyan, 1/3 mice displayed) mice injected with AAV9-FLEX-tdTomato in the FN. Raw images are registered in the Allen Mouse Brain CCF. Bottom: graphic illustration of excitatory and inhibitory FN projections in the cMAO. The region that receives excitatory FN inputs mainly consists of beta, c, and the medial part of b subnuclei, which is termed the medial cMAO (mcMAO, magenta region). The region that receives inhibitory FN inputs consists of a and the lateral part of b subnuclei, which is termed the lateral cMAO (lcMAO, cyan regions). cMAO: caudal medial accessory olive, FN: fastigial nucleus, IN: interposed nucleus, DAO: dorsal accessory olive. d, Tracing strategy (left) and representative image (right) showing AAV9-FLEX-tdTomato injected into the FN of a GlyT2-Cre mouse. Glycinergic FN neurons were selectively labeled by this approach. e, Histological sections of cMAO regions showing the absence of glycinergic FN axons. f, Glycinergic FN axons were observed in the vestibular and reticular nuclei regions of the medulla (left and middle images), as well as in the cerebellar vermis (as the nucleo-cortical mossy fibers, right image) ipsilateral to the injection site. Experiments were repeated in 3 animals. DAO: dorsal accessory olive, FN: fastigial nucleus, GRN: gigantocellular reticular nucleus, IN: interposed nucleus, LV: lateral vestibular nucleus, MV: medial vestibular nucleus, PRP: prepositus nucleus, SPIV: spinal vestibular nucleus.
