Extended Data Fig. 2: IN and DN provide exclusive inhibitory projections into distinct IO regions.
From: Excitatory nucleo-olivary pathway shapes cerebellar outputs for motor control
a, Injection strategy for labeling the excitatory projections from the IN and DN (n = 3 mice for each nucleus). b, c, Representative sections showing the injection site in the IN and the absence of VGluT2-positive axons in the IO. d, e, Same as (b, c), but for the injection in the DN. f, Same as (a), but for labeling the inhibitory projections from the IN and DN (n = 3 mice for each group). g, h, Example sections showing the injection site in the IN and dense GABAergic axonal labeling in the contralateral MAO and DAO. i, Confocal images of IN terminals in the MAO region, showing strong colocalization with the GABAergic synaptic marker VGAT, but not with the glutamatergic synaptic marker VGluT2 (n = 8 IO sections, two-sided paired t-test, P = 1.53×10−16). j-l, Same as (g-i), but for injections in the DN of Gad2-Cre mice. Dense projections from the DN were found mainly in the contralateral PO, colocalizing with VGAT staining, but not with VGluT2 staining (n = 11 IO sections, two-sided paired t-test, P = 1.03×10−22). DAO: dorsal accessory olive, DN: dentate nucleus, IN: interposed nucleus, PO: principal olive, MAO: medial accessary olive.
