Extended Data Fig. 5: Dual color retrograde labeling of VTA- or PAG-projecting MPOA Vgat+ neurons. | Nature Neuroscience

Extended Data Fig. 5: Dual color retrograde labeling of VTA- or PAG-projecting MPOA Vgat+ neurons.

From: The medial preoptic area mediates depressive-like behaviors induced by ovarian hormone withdrawal through distinct GABAergic projections

Extended Data Fig. 5

a, Schematic of injections of CTb in Vgat-Cre::Ai14 mice. b, Images showing CTb647-labeled (PAG-targeting), CTb488-labeled (VTA-targeting), and Vgat + (tdTomato expressing) neurons in MPOA. Scale bar, 100 µm. c, Percentage of MPOA Vgat+ neurons showing single and double CTb labeling (mean ± s.d., n = 3 animals).

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