Extended Data Fig. 1: The Aldh1a1+ subtype is functionally heterogeneous.
From: Unique functional responses differentially map onto genetic subtypes of dopamine neurons
(a) Schematic showing the distribution of somas and axons across the SNc and striatum for three previously described subtypes (See Poulin et al. 201828 for in depth characterization of each of these subtypes). (b) Representative distribution of somas for different subtypes within SNc. Scale bar 100 um. Thresholds for intensity scaling and gamma changes were set for each individual channel to maximize visibility of stained cells. (c) Representative projection patterns of different subtypes in striatum. Scale bar 500 um. Thresholds for intensity scaling and gamma changes were set for each individual channel to maximize visibility of stained axons. (d) Example recordings for each subtype studied (two from Aldh1a1 with different functional signaling patterns, Type 1 and Type 2), showing fluorescence traces (ΔF/F), velocity, acceleration, licking, and reward delivery times. Isosbestic control shown in blue. Large accelerations = ▲, large decelerations = ▽. (e) Cross-correlation between ΔF/F traces and acceleration for traces shown in D. Isosbestic control shown in blue. (f) Average cross-correlation between ΔF/F traces and acceleration for all recordings of each subtype and DAT (subtypes indiscriminately labeled). Isosbestic control shown in blue. Shaded regions denote mean ± s.e.m. Heatmap shows cross-correlations for each recording, sorted by the integral of the cross-correlation at positive lags. Vglut2 mice = 12, n = 42 recordings; Calb1 mice = 6, n = 22; Aldh1a1 mice = 14, n = 75 DAT mice = 14, n = 74. (g) ΔF/F triggered averages on reward delivery times for all recordings of each subtype and DAT. Isosbestic control shown in light blue, same scale as ΔF/F average. Acceleration shown in gray in the background (scale bar = 0.2 m/s2). Shaded regions denote mean ± s.e.m. Heatmap shows triggered average for each recording, sorted by size of reward response. Vglut2 mice = 11, n = 28 recordings; Calb1 mice = 8, n = 17; Aldh1a1 mice = 8, n = 30; DAT mice = 11, n = 63. (h) Distribution of locomotion response (integral of the cross-correlation at positive lags) along the dorso-ventral axis of the striatum for all recordings of all subtypes and DAT, showing how in Aldh1a1 dorsal recordings show acceleration correlation (Type 1) while more ventral recordings show deceleration correlation (Type 2). Black line represents moving average (0.5 mm bins). (i) Relationship between reward response and locomotion response for each recording of each subtype, showing how in Aldh1a1 larger reward responses correspond with deceleration correlation (Type 2), while small or negative reward responses correspond with acceleration correlation (Type 1).
