Extended Data Fig. 5: Effect of the mocha mutation on short-term plasticity in CA1 strata radiatum and lacunosum-moleculare.
a, Hippocampal region CA1 with whole cell recording electrode in the pyramidal cell layer. For VGLUT1+ inputs, stimulating electrodes were placed in stratum radiatum between regions CA3 and CA1. For VGLUT2+ inputs, stimulating electrodes were placed in stratum lacunosum-moleculare between regions CA3 and CA1. b, Representative traces from three independent experiments in response to stimulation at 1 and 25 Hz from stratum radiatum of WT and mocha slices. c, Representative traces from three independent experiments in response to stimulation at 5, 25 and 50 Hz from stratum lacunosum-moleculare of WT and mocha slices. d, Analysis of the normalized stratum lacunosum-moleculare EPSCs at 10, 30 and 90 action potentials as a function of stimulation frequency (n = 11 cells from three WT mice; n = 14 cell for 5 and 50 Hz, 9 cells for 25 Hz from three mocha mice). There is no significant difference between the genotypes by two-way ANOVA with Tukey’s multiple comparisons test. The data indicate mean ±  s.e.m.
