Extended Data Fig. 5: Hippocampal interneurons of GluN2A−/− mice had normal excitability, related to Fig. 6.
From: GluN2A mediates ketamine-induced rapid antidepressant-like responses
a–j, Representative current-clamp recording traces after injection of a 330 pA depolarizing current (a, f), quantitation of AP number (b, g), RMP (c, h), sample traces after step hyperpolarizing current injections (d, i), current-voltage curves and RIn (e, j and inserts) from pyramidal neurons of mPFC (a–e) or visual cortex (f–j) of WT (grey) or GluN2A−/− (red) mice. (mPFC: WT n = 9, GluN2A−/− n = 16; visual cortex: WT n = 13, GluN2A−/− n = 9). b, p(130 pA) = 0.012, p(150 pA) = 0.018; e, p = 0.031, p(RIn) = 0.031. k–p, Representative current-clamp recording traces after injection of a 330 (k) or 210 (n) pA depolarizing current (k, n), quantitation of AP number after depolarizing current injections (l, o), RMP (m, p) from non-fast spiking (k–m) or fast-spiking (n–p) interneurons from CA1 of WT (grey) or GluN2A−/− (red) mice. (Non-fast, WT n = 12, GluN2A−/− n = 7; Fast, WT n = 13, GluN2A−/− n = 14). 6–8 weeks old mice were used for electrophysiological recordings. Error bars show SEM. Two-way ANOVA (b, e, g, j, l, o) or Student’s t test (two-tailed) (c, e (insert), h, j (insert), m, p). * p < 0.05.
