Extended Data Fig. 6: Distribution of preparatory activity coincides with reciprocal connectivity with ALM via FN.
From: Activity map of a cortico-cerebellar loop underlying motor planning
a. Left, selectivity of individual lobules during the delay epoch in conjunction regions, input-dominant regions, and output-dominant regions. Neurons with significant selectivity during the delay epoch. Box and whisker plot shows median, 25/75th percentiles and most extreme data points not considered as outliers. Kruskal-Wallis One-Way ANOVA Test, H(2) = 12.02, P = 0.0025. Post-hoc pair-wise Mann Whitney U Test between conjunction vs. input-dominant, **P = 0.0013, conjunction vs. output-dominant, *P = 0.027. For statistical comparison between conjunction, input-, and output-dominant regions, the individual lobules in each region are combined (n = 71, 22 and 40 delay selective neurons from conjunction, input and output regions). Right, proportions of neurons with significant selectivity during the delay epoch in individual lobules. Box and whisker plot shows median, 25/75th percentiles and most extreme data points not considered as outliers (bootstrap, Methods). Χ2(1) = 7.392, ** P = 0.007, Chi-squared test. b. Left, Schematics of individual lobules. Right, population selectivity within lobule VI (output-dominant region). From left to right, schematic of Lob VI in cerebellum; ALM input-output connectivity pattern within Lob VI (same as in Fig. 3b); population selectivity (mean ± s.e.m. across neurons). Only neurons with significant selectivity during the delay epoch are used for selectivity calculation (n = 11). c. Same as b for lobule VII (conjunction region, n = 15), lobule VIII (output-dominant region, n = 12), and lobule IX (output-dominant regions, n = 3). Only neurons with significant selectivity during the delay epoch are used for selectivity calculation. Selectivity was shown as mean ± s.e.m. across neurons. d. Same as b for simplex. Dashed line, simplex lobule is subdivided into a medial portion (conjunction region, n = 17) and a lateral portion (input-dominant region, n = 2). Only neurons with significant selectivity during the delay epoch are used for selectivity calculation. Selectivity was shown as mean ± s.e.m. across neurons. e. Same as b for Crus 2. Crus 2 is subdivided into a medial portion (conjunction region, n = 29) and a lateral portion (input-dominant region, n = 3). Only neurons with significant selectivity during the delay epoch are used for selectivity calculation. Selectivity was shown as mean ± s.e.m. across neurons. f. Tessellating the cerebellum into voxels. The plots show two example voxels. Mossy fiber terminals (yellow), Purkinje cell labeling (blue), and units recorded (black dots) within each voxel. Significant selectivity during the delay epoch is indicated by gray dot. Voxels 2 shows stronger preparatory activity than voxel 1. g. Preparatory activity correlates only with conjunction of input and output connectivity. Dots, individual voxels. Only voxels containing 5 or more neurons are included. Pearson’s correlation and p values (two-sided test) are shown.
