Fig. 3: Preparatory activity is enriched in regions with conjunction of ALM input–output connectivity.
From: Activity map of a cortico-cerebellar loop underlying motor planning
a, Top, dorsal view of ALM input–output connectivity map. Data from Fig. 1. Yellow indicates ALM inputs; blue indicates Purkinje cells targeting the ALM via the FN. Bottom, map of delay epoch selectivity. Data from Fig. 2e. Color shading on the rendered brain indicates regions defined by ALM input–output connectivity: green indicates conjunction regions; yellow indicates input-dominant regions; blue indicates output-dominant regions (Methods). b, Left, normalized ALM input density (yellow) and output Purkinje cell density (blue) of individual lobules. Only lobules sampled by silicon probe recordings are shown. See Extended Data Fig. 4a for all lobules and recording yield. Simplex, Crus 1 and Crus 2 were subdivided into medial (med-SIM, med-Crus 1, med-Crus 2) and lateral (lat-SIM, lat-Crus 1, lat-Crus 2) sub-lobules based on ALM input–output connectivity. Circles indicate individual mice. Error bars denote the s.e.m. across mice. Right, ALM input density versus output Purkinje cell density for individual lobules (dots). Error bars denote the mean ± s.e.m. across mice (n = 7, ALM input labeling; n = 5 Purkinje cell labeling). c, Population selectivity (mean ± s.e.m. across neurons) in conjunction regions (n = 73), input-dominant regions (n = 20) and output-dominant regions (n = 41). Neurons with significant selectivity during the delay epoch. Selectivity is the spike rate difference between the preferred and non-preferred trial type during the delay epoch. Correct trials only. d, Schematics of individual lobules, Crus 1, PRM and Lob IV/V. e, Population selectivity within Crus 1, which has distinct patterns of ALM input–output connectivity in its subdivisions. Left, input–output connectivity pattern. Dashed line indicates the subdivision of Crus 1 into a medial portion (conjunction region) and a lateral portion (input-dominant region). Color shading is the same as in a. Bar plot, same as b but for Crus 1. Right, population selectivity (mean ± s.e.m. across neurons) of medal and lateral Crus 1. Only neurons with significant selectivity during the delay epoch (med-Crus 1, n = 12; lat-Crus 1, n = 9). f, Same as e but for population selectivity (mean ± s.e.m. across neurons) in Lob IV/V (output-dominant region). n = 15. g, Same as e but for population selectivity (mean ± s.e.m. across neurons) in PRM (input-dominant region). n = 6.
