Extended Data Fig. 8: Specific gene expression signatures of PFC and PFC subregions.
a,b, Spatial expression of representative genes enriched (Cacna1h, Cxcl12, Cdh13) or depleted (Abcd2) in PFC relative to adjacent cortical regions. Inferred expression by iSpatial is shown in b. Only excitatory neurons are shown. Corresponding ISH data from Allen Brain Atlas are shown on the right. Dotted line marks PFC region. c, Ingenuity pathway analysis (IPA) of the genes, identified after imputation, showing enriched or depleted in PFC. The red/blue bars indicate the pathway more active in/out PFC, respectively (p-values were calculated by One-sided Fisher′s Exact Test without adjustments for multiple comparison). d, The inferred spatial gene expression by iSpatial of four representative genes enriched in PFC subregions. A diagram of anatomical subregions in PFC and adjacent regions is shown on the left. Only the excitatory neurons are shown. ISH data from Allen Brain Atlas are shown on the right. Dotted line marks PFC subregion.
