Extended Data Fig. 1: The workflow and quality control for MERFISH profiling. | Nature Neuroscience

Extended Data Fig. 1: The workflow and quality control for MERFISH profiling.

From: Spatial transcriptomics reveals the distinct organization of mouse prefrontal cortex and neuronal subtypes regulating chronic pain

Extended Data Fig. 1

a, The workflow of MERFISH profiling of mouse PFC, including MERFISH imaging, decoding, segmentation and data analysis. b, Scatterplot showing the spearman correlation of the RNA counts per cell of individual genes measured by MERFISH in two independent experiments. c, Scatterplot of the RNA counts per cell of individual genes measured by MERFISH versus bulk RNA-seq data. The counts are natural logarithms. d, Spatial gene expression of three representative genes detected by MERFISH. In situ hybridization (ISH) data from Allen Brain Atlas are shown at the bottom.

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