Extended Data Fig. 6: Additional quantification of relationship between optogenetic stimulation frequency and acoustic features in anesthetized mice.
From: A brainstem circuit for phonation and volume control in mice
a, Relationship between RAm Nts optogenetic stimulation frequency and audible vocalization syllable amplitude. Data from Fig. 3e was aligned to the vocalization threshold (‘0’ on X axis indicates lowest stimulation frequency at which vocalization was elicited). Note progressive increase in peak amplitude with increasing stimulation frequency. b, Relationship between RAm Nts optogenetic stimulation frequency and audible vocalization syllable pitch for same syllables as panel a. Note lack of change in syllable pitch with increasing stimulation frequency. c, Relationship between RAm Nts optogenetic stimulation frequency and ultrasonic vocalization syllable amplitude. Data from Fig. 3f was aligned to the vocalization threshold. Note progressive increase in peak amplitude with increasing stimulation frequency. d, Relationship between RAm Nts optogenetic stimulation frequency and ultrasonic vocalization syllable pitch for same syllables as panel c. Note lack of change in syllable pitch with increasing stimulation frequency. e, Ultrasonic transition points of mice that transitioned from audible to ultrasonic vocalization with RAm Nts optogenetic stimulation. y axis indicates percent of optogenetically-driven vocalizations in the ultrasonic range (>20 kHz) at each stimulation frequency. Colored lines, individual mice (n = 6 mice). Black line, mean across all mice. Note most mice transitioned from audible to ultrasonic vocalization between 20 Hz and 30 Hz optogenetic stimulation. f, g, Quantification of peak syllable amplitude (left) and syllable pitch (right) of audible vocalizations elicited by RAm Nts optogenetic stimulation at different laser powers, while maintaining a constant pulse rate (25 Hz). Note increase in syllable loudness as laser power was increased, but lack of change in syllable pitch. Colored lines, individual mice (n = 4 mice). All r2 and p values in panels a-d calculated from simple linear regression. *: p < 0.05, **: p < 0.01, n.s.: not significant.
