Extended Data Fig. 4: Atp6v1h heterozygous deletion does not affect lysosomal acidification or degradative capacity.
From: TFEB–vacuolar ATPase signaling regulates lysosomal function and microglial activation in tauopathy
a. qPCR analysis of Atp6v1h transcripts in 9-month-old hippocampal tissues of WT and Atp6v1h heterozygous knockout (VKO). N = 4/group. b. Western blot with quantification of ATP6V1H protein levels in forebrain lysates of 9-month-old WT and VKO mice. N = 3/group. c. Representative images of LysoSensor Green DND-189 fluorescence in WT and VKO primary glial cultures. Bafilomycin (Baf) and NH4Cl treated WT cultures were used as controls. Scale bar: 10 µm. d. Quantification of (c) showing comparable levels of lysosomal acidification in VKO and WT cultures. N = 8 (WT; VKO); N = 6 (Baf); N = 7 (NH4CL). e. Representative images of DQ-BSA fluorescence co-stained with LAMP1 in WT and VKO primary glial cultures. Bafilomycin (Baf) treated WT cultures were used as a control. Scale bar: 10 µm. f. Quantification of (e) showing normal lysosomal degradation capacity in VKO cultures. N = 10/group. Data are presented as average ± SEM. Two-tailed t-test (a,b) and one-way ANOVA with Sidak’s correction (d,f).
