Extended Data Fig. 3: Cerebral endothelial cell specific Cre delivery with AAV-BR1-iCre. | Nature Neuroscience

Extended Data Fig. 3: Cerebral endothelial cell specific Cre delivery with AAV-BR1-iCre.

From: Meningeal interleukin-17-producing T cells mediate cognitive impairment in a mouse model of salt-sensitive hypertension

Extended Data Fig. 3

(A) AAV-BR1-iCre delivery in Ai14-ROSAtdTomato reporter mice demonstrates (B) widespread TdTomato (TdTM) expression in cerebral vessels. Scale bar: 500μm. (C) Specifically, we observed a 90–95% endothelial viral transduction in vessels less than 20μm (n = 5 mice, 110 vessels per mouse). (D) Representative images of TdTM expression in CD31+ endothelial cells. Scale bar: 150μm. (E) Quantification of genomic IL-17RA deletion in EC and MG (n = 4 mice/group). Intergroup differences analyzed by two-way ANOVA with Tukey’s multiple-comparison test.

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