Extended Data Fig. 5: Novel Mrc1CreERT2/+ mouse.
(A) Schematic of CNS macrophage compartments. (B) Illustration of the experimental procedure for intracerebroventricular (i.c.v.) FITC-Dextran injection and analysis of adult Mrc1+/+ or Mrc1CreERT2/+. (C-E) Immunofluorescence images reveals FITC-Dextran (green) (c) in BAMs (CD206 + , red) in perivascular macrophages (pvMΦ) and pial MΦ (D) and dural MΦ (E) compartments in the cortex of adult Mrc1+/+ or Mrc1CreERT2/+ mice. CD31+ blood vessels shown in cyan. Scale bars: 20 μm. (F) Quantification of pvMΦ, pial MΦ and dural MΦ in Mrc1+/+ (n = 4 mice) or Mrc1CreERT2/+ (n = 6 mice for of pvMΦ and pial MΦ, n = 5 mice for dural MΦ). Data shown as mean ± SEM; intergroup differences analyzed by two-way ANOVA with Bonferroni’s multiple comparison test. (G) CD206 surface expression levels on individual FITC-Dextran cells (pvMΦ Mrc1+/+ n = 586 cells, Mrc1CreERT2/+ n = 676 cells; pial Mrc1+/+ n = 235 cells, Mrc1CreERT2/+ n = 340 cells; dural Mrc1+/+ n = 1780 cells, Mrc1CreERT2/+ n = 1871 cells. Mrc1+/+ n = 4 mice, Mrc1CreERT2/+ n = 6 mice for of pvMΦ and pial MΦ, n = 5 mice for dural MΦ; 10 images per mouse per compartment, all cells within each image were analyzed). Intergroup differences analyzed by unpaired two-tailed Mann Whitney test; lines in violin plot indicate median and quartiles.
