Extended Data Fig. 7: GluN1 puncta opposes with presynaptic marker vGluT1 of glutamatergic neurons in vitro co-cultures. | Nature Neuroscience

Extended Data Fig. 7: GluN1 puncta opposes with presynaptic marker vGluT1 of glutamatergic neurons in vitro co-cultures.

From: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling

Extended Data Fig. 7: GluN1 puncta opposes with presynaptic marker vGluT1 of glutamatergic neurons in vitro co-cultures.The alternative text for this image may have been generated using AI.

(a) Immunofluorescent staining of glutamatergic presynaptic marker vGluT1 and GluN1 in coculture of neurons and aSMCs. Neuron infected by AAV2/9-CaMKIIα-EGFP virus. tdTomato+ aSMCs acquired from leptomeninges of SMACreER:Ai14 mouse pups. (b) High-resolution Airycan confocal images of adjacent locations of GluN1 and vGluT1. The specific structure enriching molecular vGluT1–GluN1 complex is defined as NsMJ, indicated by white arrows. (c) The integrated fluorescent intensity of vGluT1 and GluN1 outside and in NsMJ respectively was quantified for a and b (N = 7 cocultures of axon and aSMCs). (d) The line histogram of vGluT1 and GluN1 proteins pixel intensity along the white line in b. (e) Co-localization analysis of vGluT1 with GluN1 (N = 9 cocultures). Data are mean ± s.e.m; nested, two-tailed t-test (c, e).

Source data

Back to article page