Fig. 7: Genetic disruption of GluN1 in aSMCs attenuates vasoconstriction following neuronal SD induced by 2P optogenetics. | Nature Neuroscience

Fig. 7: Genetic disruption of GluN1 in aSMCs attenuates vasoconstriction following neuronal SD induced by 2P optogenetics.

From: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling

Fig. 7: Genetic disruption of GluN1 in aSMCs attenuates vasoconstriction following neuronal SD induced by 2P optogenetics.

a, Sequential 2P living images of p-arteriolar drastic constriction in control mouse brain (Grin1fl/fl) during an artificial SD. b, Schematic shows a recorded tdTomato+ aSMC under long-term perfusion of glutamate. c, Raw trace of mega inward current recorded from primary aSMCs, perfused with glutamate (10 mM) for 1 min at a holding potential of –40 mV (left), and summary graphs illustrate the average amplitude of the glutamate-induced inward current (right; N = 5 cells). d, Sequential 2P living images of p-arteriolar drastic constriction in aSMC-cKOGrin1 mouse brain (SMACreER:Grin1fl/fl) during an artificial SD. e, Statistical analysis of maximum arteriolar constrictions in a and d (N = 6 mice), Artificial SD was induced and imaged on the ipsilateral side of virus injection. Data are the mean ± s.e.m; nested, two-tailed t-test (e).

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