Extended Data Fig. 7: The VTADA–ACC projection modulates anxiodepression-like behaviors and persistent pain.
a, Schematic of the injection of Cre-dependent chemogenetic inhibition virus into the VTA and CNO local administration in the ACC of TH-Cre mice. b-e, Statistics of thermal pain response, EPM, DLB and CPA in the chemogenetic inhibitory and control groups (Cold/Hot plate: Control, n = 10; hM4Di, n = 7; EPM: Control, n = 8; hM4Di, n = 8 mice; DLB: Control, n = 12; hM4Di, n = 11 mice; CPA: Control, n = 8; hM4Di, n = 8 mice). f–j, As in (a-e), except that Cre-dependent chemogenetic activation virus was injected into the VTA of SNI TH-Cre mice and CPP was used for the evaluation of behavior aversion (Control, n = 12; hM3Dq, n = 13 mice; CPP: Control, n = 8; hM4Di, n = 8 mice). k-o, Schematic and statistics of pain responses and anxiety-related behaviors of sham mice with (hM3Dq) or without (Control) chemogenetic activation of VTADA-ACC projection (Control, n = 8; hM3Dq, n = 8 mice). The data are presented as the mean ± s.e.m. Unpaired Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001. Detailed statistics information can be found in the Source Data.
