Extended Data Fig. 1: Dietary fasting redistributes T cells.
a, Gating strategy used for analyzing T-cell in the blood, BM, spleen, and LN. Starting from the upper left panel and going down following the arrows in dotted lines, we gated successively: LIVE cells in R1, CD45+ cells, and T cells. T cells were subdivided into CD4+ T and CD8+ T-cell subsets. 123count eBeads were added to cell suspensions for absolute cell counting. b, Absolute cell counts of T cells in the blood (T p = 0.038, CD4+ T p = 0.021, CD8+ T p = 0.038), BM (T p = 0.0063, CD4+ T p = 0.028, CD8+ T p = 0.0059), spleen (T p = 0.013, CD4+ T p = 0.021, CD8+ T p = 0.0079), and LNs (T p = 0.024, CD4+ T p = 0.027, CD8+ T p = 0.024) of WT mice fed ad libitum or fasted for 24 hours. c, d, Percentages of dead (FVD+) T cells (c, blood p = 0.35, BM p = 0.23) and Ki67+ T cells (d, blood p = 0.71, BM p = 0.27) in the blood and BM of WT mice fed ad libitum or fasted for 24 hours. Each symbol represents an individual mouse. Mean ± s.e.m. Two-sided unpaired t-test was used in b and c. *p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant.
