Extended Data Fig. 5: Effects of Gi-DREADD activation in mice expressing p130PHR134L or p130PH and effect of Gq-DREADD activation on microglia Ca2+ and sleep.
From: Microglia regulate sleep through calcium-dependent modulation of norepinephrine transmission
a, b, Mean episode duration (a) and episode number per hour (b) for each brain state within 3 h after CNO or saline injection in mice expressing p130PHR134L. Each circle indicates data from one mouse; bars: mean ± s.e.m. (n = 8 mice). *P < 0.05, **P < 0.01 (paired two-tailed t-test; a, **P = 0.0021; b, *PNREM = 0.045; *PWake = 0.046). c, d, Similar to (a, b), but for mice expressing p130PH (n = 8 mice). e, Example imaging sessions with CNO and saline injection in mice expressing GCaMP6s and Gq-DREADD in microglia. Left, representative microglia (scale bar, 10 μm); 4 ROIs in microglia processes are outlined, whose Ca2+ traces are shown on the right. Dashed line, time of injection. f, Schematic for chemogenetic experiment with Gq-DREADD mice. g, Percentages of time in each brain state following CNO or saline injection (top, CNO: 0.2 mg/kg; bottom, CNO: 1 mg/kg; mean ± s.e.m.; n = 6 mice). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (two-way ANOVA with Bonferroni correction; top, NREM: Ptreatment < 0.0001; Ptime < 0.0001; *P0.5h = 0.015; **P1h = 0.0063; Wake: Ptreatment = 0.0043; Ptime < 0.0001; REM: Ptreatment = 0.78; Ptime < 0.0001; bottom, NREM: Ptreatment < 0.0001; Ptime < 0.0001; **P0.5h = 0.0011; **P1h = 0.0031; **P1.5h = 0.0012; *P3.5h = 0.019; Wake: Ptreatment = 0.0002; Ptime < 0.0001; *P0.5h = 0.012; REM: Ptreatment = 0.0002; Ptime < 0.0001; ****P1h < 0.0001; ***P1.5h = 0.0002). h-j, Percentage of time (h), mean episode duration (i) and episode number per hour (j) for each brain state within 3 h after CNO or saline injection. Each circle indicates data from one mouse, bars: mean ± s.e.m. (n = 6 mice). *P < 0.05, **P < 0.01, ***P < 0.001 (One-way ANOVA with Holm-Šídák’s test; h, NREM, P = 0.0029; **P = 0.0025; ***P = 0.0009; Wake, P = 0.0037; **P0.2mg/kg = 0.0072; **P1mg/kg = 0.0039; REM, P = 0.0011; *P1mg/kg = 0.011; *P0.2 vs. 1mg/kg = 0.011; i, NREM, P = 0.55; Wake, P = 0.003; *P0.2mg/kg = 0.028; **P1mg/kg = 0.0025; * P0.2 vs. 1mg/kg = 0.028; REM, P = 0.028; j, NREM, P = 0.074; Wake, P = 0.083; REM, P = 0.013; *P1mg/kg = 0.041; **P0.2 vs. 1mg/kg = 0.041).
