Fig. 6: Identification of EB R5 neurons and sleep drive correlates across EB ring neuron subtypes. | Nature Neuroscience

Fig. 6: Identification of EB R5 neurons and sleep drive correlates across EB ring neuron subtypes.

From: Single-cell transcriptomics reveals that glial cells integrate homeostatic and circadian processes to drive sleep–wake cycles

Fig. 6

a, Top, t-SNE plot of two EB ring neuron subclusters. Bottom, reclustering of EB ring neurons into three subclusters, that is, ring_A, ring_B and ring_C. b, Top 20 DEGs between the three subclusters. c (i)–(v), Expression pattern and chemical tag staining of T2A-Gal4 driver lines of the genes boxed in b. Their morphology revealed that neurons in ring_C are EB R5 neurons. d, t-SNE displaying the number of sleep drive correlates at three-cluster resolution. e, Cluster map displaying the expression of sleep drive correlates for R5 neurons. A subset of significantly correlated sleep drive genes is labeled. f, t-SNE displaying the number of sleep drive correlates at a nine-cluster resolution.

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