Fig. 7: Analysis of sleep drive correlates in clock neuron subtypes.

a, Predicted annotations of clock neuron subtypes on our data based on training the scANVI model on data from Ma et al.²². Cluster annotation names were transferred from Ma et al.²². b, VGlut expression mapped onto our data; DN1p subtype clusters 18 (green) and 4 (red) are indicated. c, Plotting of our 494 clock neurons together with clock scRNA-seq atlas of Ma et al.²² split into 17 high-confidence annotated clusters. d, Our cells in blue highlighted in the UMAP space with data from Ma et al.²². e, Proportion of cells according to clock subtype for data from Ma et al.²², data from this study and published counts of cells from neuroanatomical studies. f–h, Cluster map of all significant sleep drive correlates of 18:DN1p (f). Gene expression of the significant genes for 18:DN1p did not correlate with sleep drive in another DN1p subtype (g) or 14:DN3 (h).