Fig. 8: Homeostatic and circadian processes converge on glial cells.
a, Clusters assigned to cyclers (yellow, left) or sleep drive correlates (blue, right) visualized in the t-SNE. Middle, The t-SNE shows the merge of left and right t-SNE, highlighting the clusters assigned to both groups in green. b, Number of correlating genes with circadian or sleep drive template across all annotated clusters that have correlates for either process. The color indicates their assigned group (yellow, cyclers; blue, sleep drive correlates; green, both). c, Sleep amount 4 h after SD compared to the baseline sleep of the same fly in the same ZT time period before SD in flies expressing conditional knockout of vri (n = 30), tim (n = 47) and cry (n = 35) in pink and control flies (repo-Gal4>iso31 (n = 58); repo-Gal4>UAS-Cas9.P2 (n = 30); repo-Gal4>UAS-sgRNA-vri (n = 41); repo-Gal4>UAS-sgRNA-tim (n = 26); and repo-Gal4>UAS-sgRNA-cry (n = 41)) in purple. d, Sleep amount 4 h after SD compared to baseline sleep of the same fly in the same ZT time period before SD of flies expressing a dominant negative form of Cyc (n = 48) in pink and control flies (repo-Gal4>iso31 (n = 58)) in purple. c,d, The statistical method used was a Wilcoxon rank-sum test, with Bonferroni-corrected P value adjustment for multiple-comparisons testing. The boxplots indicate the minimum, median, maximum, and first and third quartiles. The error bars represent the first (third) quartile ± 1.5 times the interquartile range. Adjusted *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant.
