Extended Data Fig. 8: Representative images and specificity of the labeling approaches used in mouse experiments.

a, Representative images of experimental approaches. Left, Cal-Light labeling in Fig. 3a. Middle, CCK⁺ neuronal eArch3.0-eYFP labeling in Fig. 5e. Right, CCK⁺ neuronal hM4Di-mCherry labeling in Fig. 6e. For each experiment, representative image of a group of 3 independent samples. b-c, Means and standard deviations are shown. b, Proportion of PV⁺ cells, CCK⁺ cells, and granule cells (GC⁺) in c-Fos⁺ populations. Fluorescent in situ hybridization (FISH) was used to determine the proportion of PV⁺ vs. GC⁺ cells (top row, n = 3 mice) and CCK⁺ vs. GC⁺ cells (bottom row, n = 4 mice) in c-Fos⁺ populations that were induced by contextual fear training. c-Fos⁺ labeling within the GC layer that was PV^- and CCK^- represents GC⁺ counts. Left, representative images. Right, proportion of cells in c-Fos⁺ populations. c, Specificity of AAV₅-Dlx5/6-DIO-eArch3.0-eYFP in CCK-Cre mice (from Fig. 5e). FISH was used to measure the proportion of the virally-labeled CCK⁺ neurons (eYFP probe) that overlapped with endogenous CCK mRNA (top row) and endogenous GAD1 mRNA (bottom row). n = 3 mice per group.

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