Fig. 3: Dynamic and selective engrams encode contextual fear memory.
From: Dynamic and selective engrams emerge with memory consolidation
a, Schematic of experimental protocol with Cal-Light labeling of fear training-activated neurons (Methods). b–d, Means and 99% confidence intervals are shown. b, Freezing levels during memory recall in a as a function of delay time. Two-sided Wilcoxon signed-rank test between freezing in the training and neutral contexts. n = 11 mice per group. c, Discrimination index between freezing levels in the training and neutral contexts in b as a function of delay time (Methods). Two-sided one-sample Wilcoxon signed-rank test against discrimination = 0. n = 11 mice per group. d, Ensemble overlap between neurons activated during both recall and training (c-Fos+ ∩ EGFP+) as a fraction of training-activated neurons (EGFP+) (left), recall-activated neurons (c-Fos+) (middle) and all cell counts (DAPI+) (right) in a (Methods). One-way ANOVA followed by Tukey’s HSD post hoc test. n = 6 mice per group. e, Schematic of experimental protocol with optogenetic reactivation of Cal-Light-labeled neurons (Methods). f, Freezing levels when mice were placed in the neutral context in e as a function of delay time. Means and 99% confidence intervals are shown. Unpaired t-test between the control and the Cal-Light activation groups. n = 9 mice per group. b–d,f, *P < 0.05; NS, not significant. For detailed statistical test results, see Supplementary Table 1.
