Extended Data Fig. 1: Functional assessment of glucose sensor in OLs.
From: Oligodendrocyte–axon metabolic coupling is mediated by extracellular K+ and maintains axonal health
a, AAV-mediated glucose FRET sensor FLIIP expression in optic nerve OLs of wildtype mice. Depicted are representative color-coded ratio images from optic nerve in ACSF containing 10 mM glucose (Glc), after removal of extracellular Glc, and following inhibition of glycolysis by 1 mM iodoacetate (IA) in ACSF containing 10 mM Glc. Warm and cold colors indicate high and low ratios or glucose levels, respectively. Scalebar 20 µm. b, Quantification of normalized ratios obtained from conditions presented in a. Ratios (n = 3 mice) were normalized to the averaged minimum obtained at 0 mM [Glc]O. Data shown in mean ± SEM.
