Extended Data Fig. 6: Anterior rather than posterior PBNslCck+ neurons contribute to odor-driven innate fear but not in visually and auditorily evoked innate fear.
a, Typical images of tdT-labeled neurons co-expressed with Fos and co-labeled percentage in anterior PBNsl. b, Typical images of Fos expression in posterior PBNsl and quantification of Fos number. c, Typical image of Fos co-labeled with tdT in posterior PBNsl, and percentage quantification. d, Schematic of virus injection and fiber implant, typical image of anterior PBNslCck+ neurons expressing GCaMP6s, and representative traces of calcium signals. e-h, Schematic of virus injection (e), average calcium signals / heatmap (f), multicurve presentation (g), peak delta F/F and AUC of calcium activity of anterior PBNslCck+ neurons (h) in mice exposed to TMT and water and DP-lesioned mice with TMT treatment. i, Schematic of virus injection, typical image of posterior PBNslCck+ neurons expressing GCaMP6s, and average calcium signals of TMT stimuli. j & l, Average calcium signals of mice under escape, frozen, and unresponsive conditions for looming (j) and auditorily (l) evoked innate fear. k & m, Peak delta F/F and AUC of calcium activity for looming (k) and auditorily (m) evoked innate fear. n, Schematic of virus injection, typical image of anterior PBNslGad2+ neurons expressing GCaMP6s, and average calcium signals. o, Heatmap of calcium transients of natural fox urine, male mice urine and peanut oil stimulations. p, Percentage of neurons excited (red), inhibited (blue), or with no response to odor (gray). q, Anterior to posterior brain slices from mouse following GCaMP6s virus injection into anterior PBNsl. r & s, Overlay of GCaMP6s virus expression (green), fibers (blue) and lens (yellow) positions. For all the typical images, Scale bar, 100 μm; Zoom, 50 μm. All statistical tests are two-tailed. For (a)-(c), n = 5 brain slices from 3 mice per group; (h), (k), and (m), n = 10 trials from 5 mice. Unpaired t test for (a) and (b); One-way ANOVA with bonferroni post hoc test for (h), (k), and (m). *p < 0.05; **p < 0.01; ****p < 0.0001; N.S., not significant. Data are presented as mean ± s.e.m. See Extended Source Data Fig. 6 for more detailed statistics.
