Extended Data Fig. 7: Effects of anterior PBNslCck+ neuron ablation on TMT-induced innate fear and virus targeting.
a, Average speed of opto-silencing of PBNslCck+ neurons for open field test. b, Representative trajectory chart of PBNslCck+ neurons for day 1 laser OFF tests. c, Average escape speed curves (dotted line indicates the point when mice exit from area 1). d, Schematic of virus injection, typical images of taCasp3-induced apoptosis, and number of anterior PBNslCck+ neurons. Scale bar, 100 μm. e–k, Representative trajectory chart (e), average escape speed curves (f), escape speed (g), escape percentage (h), time in area 1 (i), aversion index (j), and freezing time percentage (k). l, Typical images of pupil. m, Average curves and summarized data of normalized pupil size. n, Average curves and summarized data of normalized heart rate. o & p, Anterior to posterior brain slices from mouse following NpHR-EYFP (o) or ChR2-EYFP (p) virus injection into anterior PBNsl and overlay of virus expression. Scale bar, 200 μm. All statistical tests are two-tailed. For (a), n = 12 mice per group; (d), n = 6 brain slices from 3 mice per group; For (g)-(k), n = 11 mice per group; For (m), n = 10 mice for EYFP group / n = 13 mice for ChR2 group; For (n), n = 10 mice for EYFP group / n = 12 mice for ChR2 group. A repeated two-way ANOVA measures with bonferroni post hoc test for (a); Unpaired t test for (d), and (g)-(k). Repeated one-way ANOVA measures with bonferroni post hoc test for (m) and (n). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; N.S., not significant. Data are presented as mean ± s.e.m. See Extended Source Data Fig. 7 for more detailed statistics.
