Extended Data Fig. 10: Effects of ablation of specific amygdala nuclei on DP→anterior PBNsl→PSTh circuit-triggered innate fear and pain threshold.
a, Average speed of opto-silencing of DP→anterior PBNsl→PSTh pathway for open field test. b, Representative trajectory chart of DP→anterior PBNsl→PSTh pathway for day 1 laser OFF tests. c, Average escape speed curves (dotted lines indicate the point when mice exit from area 1). d, Schematic of virus injection and strategy for optogenetic manipulation of DP→anterior PBNsl→PSTh triple pathway. Symbol “&” means “and”. e, Typical images of pupil. f & g, Average curves (f) and summarized data of normalized pupil size (g). h & i, Average curves (h) and summarized data of normalized heart rate (i) amygdala-ablated mice when triple pathway activated. j & k, Anterior to posterior brain slices from mouse following Cre/Flp-dependent virus expressing NpHR-EYFP (j) or ChR2-EYFP (k) injection into anterior PBNsl and overlay of virus expression. Scale bar, 200 μm. l, Schematic of pain threshold tests including von Frey and Hargreaves test. m & n, Effects of activation of triple pathway on mechanical (m) and thermal (n) thresholds. All statistical tests are two-tailed. For (a), (m), and (n), n = 12 mice per group; For (g), n = 10 mice for EYFP group / 12 mice for ChR2 group and ChR2 + lesion group; For (i), n = 10 mice for EYFP group / 12 mice for ChR2 group and ChR2 + lesion group. A repeated two-way ANOVA measures with bonferroni post hoc test for (a), (m), and (n); A repeated one-way ANOVA measures with bonferroni post hoc test for (g) and (i). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; N.S., not significant. Data are presented as mean ± s.e.m. See Extended Source Data Fig. 10 for more detailed statistics.
