Extended Data Fig. 5: Automated clustering of spatial sequencing.

Heat map of the top 5 most expressed genes per cluster of integrated spatial sequencing data from all sequenced samples. The color represents normalized and scaled (‘SCTtransform’, Seurat) gene expression (a). These unsupervised clusters are distributed similar to the supervised anatomical annotation shown in Fig. 3 with clusters of brainstem nuclei and white matter fiber tracts (b; left panel: acute (patient D10), right panel: late (patient D19)).