Extended Data Fig. 9: RGFP decreases gene expression associated with reactive astrocytes in ONC.
From: A phenotypic screening platform for identifying chemical modulators of astrocyte reactivity
a, Bulk RNA-seq volcano plot of genes up and downregulated in ONC retina versus naïve retina. log2(FC) and p-adj values were generated from bulk RNA-seq analysis with DESEQ2. b, Gene ontology terms for enriched for genes upregulated in ONC retina versus naïve retina. P-values generated by Benjamini-Hochberg false discovery rate. c, Tukey box and whisker plot depicting how the expression of genes upregulated in ONC retina change between crushed and naïve retina in mice treated with vehicle and mice treated with 10 mg/kg RGFP. Data are presented as log2(FC) for n = 7 biological replicates. P-value generated by a paired two-tailed t-test. d, Tukey box and whisker plot depicting the log2(FC) expression of ONC reactive astrocyte genes (same as in Fig. 5k) between naïve retina treated with 10 mg/kg RGFP and naïve retina treated with vehicle. Data are presented as log2(FC) for n = 7 biological replicates. P-value generated with a one-sample Wilcoxon signed ranked test comparing to a hypothetical median of log2(FC) = 0, which would designate no difference in expression. e,f, Representative images and quantification of retinal ganglion cells (RGCs) double stained with BRN3A (green) and beta-3 tubulin (red) in retina from naive mice treated with vehicle or 10 mg/kg RGFP966. Scale bar is 20 µm. Data are mean ± s.e.m., n = 9 biological replicates (mice), p-value by unpaired two-tailed t-test.
