Extended Data Fig. 7: The Yoda1 Effect on Brain Fluid Outflow Persists Up to 24 Hours.
From: Piezo1 regulates meningeal lymphatic vessel drainage and alleviates excessive CSF accumulation
Adult wild-type lymphatic reporter mice (Prox1-tdTomato, 9-10 months old) were i.p. injected with the vehicle or Yoda1 (213 µg/kg) at seven days, 24 hours, 12 hours before tracer i.c.m. injection (OVA-Green: Ovalbumin, Alexa Fluor™ 488 Conjugate). After 45 minutes post tracer injection, LNs were collected and imaged to visualize the drained tracer (a). (b, c) The relative tracer intensity in the cervical LNs (b) and mandibular LNs (c) was quantified (n = 6 mice/group). Each data point represents the sum of the fluorescence intensity of LNs on the right and left sides of a single mouse. Scale bars: 500 µm. Statistics: Kruskal-Wallis H test, p = 0.0032 (b); p = 0.0002 (c), followed by Bonferroni correction method (statistical significance, p < 0.0083). Data are presented as mean values +/- SEM.
