Extended Data Fig. 9: Piezo1 Activation Decreases the CDH5-stained Area and Increases Drainage of Lymphatic Vessel Mimetics in Polydimethylsiloxane (PDMS) Chip.
From: Piezo1 regulates meningeal lymphatic vessel drainage and alleviates excessive CSF accumulation
(a) Schematic illustration of the 3-D lymphatic vessel model used for this study. The outcome of the Yoda1-induced drainage increase is presented in Fig. 4c. (b) Fluorescence confocal images of the engineered lymphatic vessels stained for F-actin and CDH5. Enlarged CDH5 images (boxed) show more discontinuous junctions in the Yoda1-treated group than in the vehicle group. Scale bars: 100 μm (10 μm, enlarged images). Acellular channels are not shown. (c) The relative area of CDH5-stained cellular junctions (n = 8 independent experiments). Statistics: two-tailed t-test. (d) Western blot assays verifying the efficient knock-down of Piezo1 in LECs prepared for the drainage measurement shown in Fig. 4c (n = 4 independent samples). Data are presented as mean values +/- SEM.
