Extended Data Fig. 4: Relationship of mRNA sets depleted in BORCS5-KO axons to neurodegenerative disorders and COVID-19, and GO Cellular Component and KEGG pathway analysis of changed mRNA sets.

a, Venn diagram for functional enrichment related to neurodegenerative disorders. DEGs that were down in BORCS5-KO axons compared to WT axons matched to gene sets related to neurodegenerative disorders, as defined by KEGG. b, Venn diagram for functional enrichment related to ribosomes and COVID-19. DEGs that were down in BORCS5-KO axons compared to WT axons matched to gene sets related to ribosome and COVID-19, as defined by KEGG. c, GO Cellular Component analysis of gene sets that are increased or decreased in BORCS5-KO neurons vs WT neurons and BORCS5-KO axons vs BORCS5-KO neurons. d, KEGG pathway analysis of gene sets that are increased or decreased in BORCS5-KO neurons vs WT neurons and BORCS5-KO axons vs BORCS5-KO neurons. Both c and d show dot plots for DEG sets that are increased (up) or decreased (down) in RNA-Seq. Enriched terms are arranged by statistical significance (FDR), showing the top 12 terms. The z-score captures both the direction of changes and the number of genes changing in each direction. A larger absolute z-score indicates a more biased direction towards increase or decrease. Statistical significance was calculated by one-sided Fisher’s exact test. P-values were adjusted for multiple comparisons using the Benjamini-Hochberg method.