Extended Data Fig. 8: Tau aggregates and microtubule swirls in axonal swellings from BORCS7-KO i3Neurons. | Nature Neuroscience

Extended Data Fig. 8: Tau aggregates and microtubule swirls in axonal swellings from BORCS7-KO i3Neurons.

From: Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration

Extended Data Fig. 8

a, WT and BORCS7-KO i3Neurons were cultured for 25 days on glass coverslips and stained with Tau-1 antibody (marker for axons and axonal degeneration when aggregated), TOMM20 (mitochondria), and MAP2 (soma and dendrites). Nuclei were stained with DAPI (blue). Neurons were imaged by confocal fluorescence microscopy. Notice that swellings in BORCS7-KO axons (yellow arrows) contain Tau-1-positive aggregates and mitochondria. Scale bars: 10 μm. Experiments were repeated three times. b, BORCS7-KO i3Neurons grown for 25 days on glass coverslips were analyzed by TEM. Left column: detailed view of two axonal swellings in BORCS7-KO axons. Right column: magnified views of the boxed areas on the left. Curved microtubules (MT) indicative of a swirl-like organization are visible in the swellings (arrows). Scale bars: 400 nm. This experiment was repeated twice.

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