Extended Data Fig. 9: Axonal swellings containing neurofilament protein, Tau, and autophagosomes in sciatic nerves from BORCS5-KO mice. | Nature Neuroscience

Extended Data Fig. 9: Axonal swellings containing neurofilament protein, Tau, and autophagosomes in sciatic nerves from BORCS5-KO mice.

From: Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration

Extended Data Fig. 9

a, b, Sciatic nerves were isolated from WT and BORCS5-KO E17 mouse embryos. Nerves were fixed and immunostained for endogenous Tau (magenta) and LAMP1 (lysosomes, green) (panel a) or LC3B (autophagosomes, grayscale) (panel b). Nerves were also co-stained for NFH (axons, grayscale in panel a and magenta in panel b), and DAPI (nuclei, blue), and imaged by confocal fluorescence microscopy. Enlarged views of the boxed areas are shown on the right. Areas encircled by dashed lines indicate swellings devoid of LAMP1 staining. Scale bars: 20 μm. c, Quantification of the number of axonal swellings (NFH-positive) per unit of sciatic nerve area from n = 9 images captured from n = 3 animals. Values are the mean ± SD from representative images like those shown in panels a and b. Statistical significance was calculated using an unpaired two-tailed Student’s t test. BORCS5 KO vs WT ***P < 0.001.

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