Extended Data Fig. 10: Accumulation of autophagosomes in the axons of BORCS5 -KO mouse brain. | Nature Neuroscience

Extended Data Fig. 10: Accumulation of autophagosomes in the axons of BORCS5 -KO mouse brain.

From: Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration

Extended Data Fig. 10

a, Brains were harvested from WT and BORCS5-KO E17 mouse embryos and the corpus callosum was analyzed by TEM as described in the Methods. The figure shows low magnification (left column) and high magnification images of the boxed areas (right column). Notice the presence of numerous autophagosomes (AP) in the axons of BORCS5-KO neurons, morphologically identified by their size and presence of synaptic vesicles, microtubules, and neurofilaments. Scale bars: 600 nm. b, Quantification of the number of autophagosomes per field unit area from n = 25 images captured from n = 2 animals. Values are the mean ± SD from representative images like those shown in panel a. Statistical significance was calculated using an unpaired two-tailed Student’s t test. BORCS5 KO vs WT ***P < 0.001.

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