Extended Data Fig. 4: Effect of VTA dopaminergic neuron inhibition on spontaneous striatal activity.
From: Constraints on the subsecond modulation of striatal dynamics by physiological dopamine signaling
a. Top: Experimental approach. Bottom: Task schematic. b. dLight fractional fluorescence change (mean ± SEM) from one eNpHR3-expressing mouse and one opsin-free control mouse. c. Mean laser-evoked dLight fluorescence (n = 5 eNpHR3 and 3 control mice, unpaired two-sided t-test, t = 4.2, **P = 0.005). d. Total percentage of neurons significantly modulated by laser relative to baseline (n = 46 out of 589 cells in eNpHR3 group, n = 25 out of 344 cells in control group, chi square test, χ2 = 0.09, P = 0.8, df = 1). e. Maximum selectivity index per neuron (n = 589 cells in the eNpHR3 group, n = 344 cells in the control group, unpaired two-sided t-test, t = 1.1, P = 0.3). Selectivity of neural activity is calculated relative to baseline. Lines represent mean ± SD, dots represent individual cells. f. Firing rate changes (mean ± SEM) by cell type (eNpHR3 animals: n = 435 MSN, 73 FSI, 58 TAN; control animals: n = 261 MSN, 44 FSI, 25 TAN; one-way ANOVA, cell type x group effect: F5,890 = 4.303, P = 0.0007). Post-hoc Sidak’s test comparing eNpHR3 and controls: MSN (P = 0.78), FSI (P = 0.45), TAN (P = 0.37). g. Top: Selectivity index of 589 neurons pooled across 5 eNpHR3-expressing mice, and 344 neurons pooled across 3 control mice. Bottom: Percentage of neurons significantly modulated by the laser relative to baseline. h. Mean accuracy of SVM decoder trained using 50 neurons to discriminate laser-evoked from baseline activity. Magenta dashed line indicates the 95 % confidence interval of decoder performance trained on trial-shuffled data. Top: eNpHR3 group. Bottom: control group. Shaded area represents the SD across 50 random drawings of neurons. i. Maximum decoder accuracy (mean ± SD across 50 drawings) by neuron number (two-way ANOVA, group effect: F1,784 = 8, P = 0.004, neuron number effect: F7,784 = 116, P < 0.0001). Post-hoc Sidak’s test: **P = 0.005 for n = 50 neurons; other comparisons between eNpHR3 and control group not significant.
