Extended Data Fig. 6: Effect of activating VTA GABAergic neurons on spontaneous striatal spiking activity.
From: Constraints on the subsecond modulation of striatal dynamics by physiological dopamine signaling
a. Top: Experimental approach. Bottom: task schematic in which 0.5 s pulsed laser stimulation is delivered in the absence of reward or other stimuli. b. Top: dLight fractional fluorescence change signal during laser stimulation from one Vgat-Chrimson mouse. Bottom: Mean firing rate of one neuron. Data represent mean ± SEM. c. Top: Selectivity index of 379 neurons pooled across 3 Vgat-Chrimson mice. Bottom: Percentage of neurons that were significantly modulated by the laser relative to baseline, as a function of time. d. Top: Mean accuracy of an SVM decoder trained using 50 neurons to discriminate laser-evoked from baseline activity. Shaded area represents the SD across 50 random drawings of neurons. Magenta line indicates the 95 % confidence interval of decoder performance trained on trial-shuffled data. Bottom: Maximum decoder accuracy as a function of neuron number (one-way ANOVA, neuron number effect: F7,392 = 441, P < 0.0001). Post-hoc Sidak’s test: P < 0.0001. Data represent the mean and SD across 50 random drawings of neurons.
