Extended Data Fig. 4: GnRHOB neurons are preferentially activated by female urine.
a, Representative in vivo calcium-imaging responses of a cell to female urine (FU), saline (Sal) and male urine (MU). Three consecutive trials for each stimulus are represented. Dashed lines indicate stimulus onset. The displayed cell (cell ID = 38) was classified as a responsive cell to FU but not for Sal and MU. b, Quantification of cell responsiveness to Sal (n = 29 cells recorded from 13 mice; paired two-sided t test). Left: average fluorescent signal across all recorded cells in response to Saline (dark gray) or fresh air (gray). Right: quantification of peak fluorescence in the 10 s after stimulus onset for Sal or Air. Gray dots and lines indicate single recorded cells. Black dots and line show the mean and the associated standard error (paired two-sided t test, n = 29 cells). c, Same as in b but for responses to MU (n = 19 cells recorded from 9 mice) (paired two-sided t test). d, Same as in b but for responses to methyl butyrate, n = 7 cells (paired two-sided t test). P values are shown in the figure.
