Extended Data Fig. 10: Bace1 cKO does not result in changes to neuronal numbers, axonal densities, and myelination profile.
(a) Closeups of fluorescence microscopy images of cortices of controls and cKOs highlighting unchanged density of neurons and axons in both cKO lines at a gross level. (b) Quantification of neuronal and axonal densities between 6-month-old female controls and cKOs (n = 5 per group) in the isocortex. (c-g) Bace1 cKO does not alter myelination profile. (c) Closeups of fluorescence microscopy images of cortices of controls and cKOs highlighting unchanged density of OLs and myelination in both cKO lines at a gross level. (d) Quantification of OL density between controls and cKOs (n = 5 per group) spanning different regions. (e) Quantification of myelin density between controls and cKOs (n = 5 per group) spanning different regions. As CC and alveus are densely myelinated tracts, mean intensity values were instead measured. (f) Representative electron micrographs of caudal corpus callosum (CC) of controls and cKOs at 6 months of age. (g) Analysis of myelin thickness via g-ratio measurement with single dots representing single myelinated axons quantified (Control;AD = 397, OL-Bace1cKO;AD = 417, ExN-Bace1cKO;AD = 394). Lines represent linear regressions of each group and shaded area indicates error bars. Myelinated axon counts and mean g-ratio comparisons from electron micrographs of controls and cKOs (n = 4 per group). For (b,d,e,g), one-way ANOVA was performed with Tukey multiple comparison tests (P values indicated in graphs with significance highlighted in bold). Bars represent means with SEM and individual data points displayed.
