Extended Data Fig. 2: OLs exhibit protein expression of APP and an abundance of APP and BACE1 transcripts.
(a) A confocal image of an in vitro OL with APP present in the soma and cellular processes. Experiment was repeated thrice in separate in vitro cultures. (b) A confocal image of in vivo mouse cortical OLs with APP present in the perinuclear space. Experiment was repeated thrice in separate in vitro cultures. (c) A fluorescence microscopy image of human cortical OLs showing APP reactivity in the perinuclear space. Immunolabeling was performed once on all control human samples (n = 3). (d) ISH images of human cortical layers 5–6 with visible APP puncta in MBP+ cells of control (left) and AD (right) patients. Arrowheads point to APP-expressing OLs. (e) ISH images of human cortical layers 5-6 with visible BACE1 puncta in MBP+ cells of control (left) and AD (right) patients. Arrowheads point to BACE1-expressing OLs. (f) Quantification of nuclear count, MBP+ nuclei, MBP+ APP+ nuclei, and MBP+ BACE1+ nuclei in control (n = 3) and AD (n = 3–4) patients. One-way ANOVA was performed with Sidak multiple comparison tests (P values indicated in graphs with significance highlighted in bold) comparing AD patients to controls. Bars represent means with SEM, and individual data points are displayed.
