Extended Data Fig. 4: ISH validation of Bace1 cKO in OL-Bace1cKO;AD and ExN-Bace1cKO;AD.
(a-d) ISH validation of Bace1 cKO in cortical ExNs. (e-g) ISH validation of Bace1 cKO in fimbria OLs. (h-m) ISH validation of Bace1 cKO in hippocampal ExNs. (a) Fluorescence microscopy images of cortices showing reductions of Bace1 transcripts in ExNs of ExN-Bace1cKO;AD samples. (b) Violin plots showing individual mouse distribution of Bace1 puncta/cortical ExN nuclei. (c) Frequency distribution heatmap of individual mouse distribution of Bace1 puncta/cortical ExN nuclei. (d) Violin plots showing cortical layer-specific individual mouse distribution of Bace1 puncta/ExN nuclei. (e) Fluorescence microscopy images of hippocampal fimbriae showing reductions of Bace1 transcripts in OLs of OL-Bace1cKO;AD samples. (f) Violin plots showing individual mouse distribution of Bace1 puncta/fimbria OL nuclei. (g) Frequency distribution heatmap of individual mouse distribution of Bace1 puncta/fimbria OL nuclei. (h-j) Fluorescence microscopy images of hippocampal CA1, CA2/3, and DG respectively showing reductions of Bace1 transcripts only in ExNs of ExN-Bace1cKO;AD samples. (k) Violin plots showing individual mouse distribution of Bace1 puncta/hippocampal ExN nuclei. (l) Frequency distribution heatmap of individual mouse distribution of Bace1 puncta/hippocampal ExN nuclei. (m) Violin plots showing hippocampal region-specific individual mouse distribution of Bace1 puncta/ExN nuclei. For (b,d,f,k,m), comparison was made between control, OL-Bace1cKO;AD, and ExN-Bace1cKO;AD mice (n = 2 per group). Solid lines represent median and faded lines represent quartiles. For (c,g,l), n-numbers refer to amount of nuclei considered for each region and cell type analysis and make up the data cloud for violin plots shown in (b,d,f,k,m).
