Extended Data Fig. 1: Morphological, molecular and functional differences of the vasculature between the ME and cortex. | Nature Neuroscience

Extended Data Fig. 1: Morphological, molecular and functional differences of the vasculature between the ME and cortex.

From: Characteristics of blood–brain barrier heterogeneity between brain regions revealed by profiling vascular and perivascular cells

Extended Data Fig. 1

(a) Tracer leakage assay with Sulfo-NHS-Biotin (magenta) and immunostaining for blood vessels (CD31, white) in cortex (upper panel) and ME (lower panel). Tracer in circulation was washed out by perfusion prior to analysis. Scale bar 10 µm. (b) Co-immunostaining of GLUT1 (green) and PLVAP (red) in ME and cortex. Scale bar 100 µm. (c) High magnification images of capillaries showing distinct Glut1 (green) and Plvap (red) expression pattern and vessel morphology in the cortex and ME. Scale bar 20 µm. (d) Co-immunostaining of CD31 (white) and tight junction protein Cldn5 (green) in cortex and ME. Scale bar 10 µm. (e) Validation of specificity of newly generated polyclonal antibody against MFSD2A (green) by immunostaining of cortex from wild type and Mfsd2ako mice. Scale bar 100 µm. (f) Immunostaining of CD31 (white) in the ME in thick tissue section. Scale bar 100 µm.

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