Fig. 4: Measurement of dendritic spines with protein-based functional characterization. | Nature Neuroscience

Fig. 4: Measurement of dendritic spines with protein-based functional characterization.

From: Integration across biophysical scales identifies molecular and cellular correlates of person-to-person variability in human brain connectivity

Fig. 4

a, Representative ×60 bright-field image of a Golgi-stained dendrite from ITG of an exemplar participant. Scale bar = 10 µm. b, Digital 3D reconstruction of the dendritic segment performed on the bright-field image. c, Digital 3D reconstruction used for estimating spine density and morphometric attributes, including head diameter, length and volume, and assigning subclasses. Blue indicates thin spines, green indicates mushroom spines, red indicates stubby spines and yellow indicates filopodia. d, Representative zoomed-in bright-field image of a single Golgi-impregnated thin spine in the xy plane (red box from c). e, Left to right, 3D digital reconstruction of the dendrite (gray) and spine (green) in the xy plane, clockwise rotation in xyz dimensions and further rotation in xyz. f, Each spine attribute was associated against all proteins measured from the same region with enriched GO terms indicated (SFG in green and ITG in blue). g, T values of contrasts between SFG and ITG for each spine morphologic attribute are shown, with * indicating nominal differences.

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