Extended Data Fig. 9: Intrarenal injection of α-Syn PFFs promotes α-Syn pathology in wild-type mice.
From: Propagation of pathologic α-synuclein from kidney to brain may contribute to Parkinson’s disease
a,b, Immunohistochemistry of pα-Syn in the kidney, spinal cord, locus coeruleus (LC), substantia nigra compacta (SNc), basolateral amygdala (BLA), hippocampus (HIP), striatum (STR), cortex (CTX) and olfactory bulb (OB) of wild-type mice that received intrarenal injection of PBS or α-Syn PFFs (P = 0.0124 (kidney, PFFs 6m vs. RD + PFFs 6m), P = 0.0002 (STR), P < 0.0001 (kidney: PFFs 6m vs. PBS 6m, spinal cord, LC, SNc, BLA, HIP, CTX, OB)). Arrowheads: pα-Syn-positive signals. c, Western blot analysis of pα-Syn in the RIPA-soluble and RIPA-insoluble fractions of the spinal cord (left) and cortex (right) of wild-type mice injected with PBS or α- PFFs, respectively (P < 0.0001). Error bars indicate the mean ± s.e.m. *P < 0.05, ***P < 0.001. n = 5 mice per group, one-way ANOVA was used. AU, arbitrary units; RD, renal denervation. Scale bars: 20 μm.
