Extended Data Fig. 2: The kidneys physiologically remove α-Syn from the blood.
From: Propagation of pathologic α-synuclein from kidney to brain may contribute to Parkinson’s disease
a,b, Mice with normal kidney (a) or renal failure (b) were intravenously injected with recombinant human α-Syn PFFs. The concentrations of human α-Syn in the serum and urine were determined at different times after injection. c, Total α-Syn in the 24-hour urine of mice injected with α-Syn or PFFs (c) (n = 5 mice per group, P < 0.0001 (control PBS vs. control α-Syn, renal failure PBS vs. renal failure α-Syn), P = 0.5176 (control α-Syn vs. renal failure α-Syn), error bars indicate the mean ± s.e.m; ns, not significant; ***P < 0.001, two-way ANOVA). d, Immunohistochemistry showing the overall distribution of human α-Syn in the kidney at 30 min after intravenous injection of human α-Syn monomers. e, Immunohistochemistry of human α-Syn in different organs of mice without renal failure at different time points after intravenous injection of recombinant human α-Syn monomers. f, Immunohistochemistry of human α-Syn in different organs of mice with renal failure at different time points after intravenous injection of recombinant human α-Syn monomers. Scale bars: 100 μm (d) and 20 μm (e,f).
