Fig. 7: The vLGN activity is necessary for the coordination of behaviors and arousal.
a, Schematic of bilateral viral TeLC expression in Gad2+ neurons in the vLGN/IGL complex. b, Schematic of optokinetic reflex paradigm. c, Top, sorted eye positions for control and TeLC animals in trials where animals were not locomoting. Each trial is zeroed to the starting position. Note the smooth appearance in TeLC animals compared with controls, indicating absence of saccades. Bottom, average eye position plotted next to the stimulus displacement. d, Top, saccadic events (displacements > 2.5° per frame), trial sorted as in c. Bottom, normalized saccadic counts for temporal and nasal saccades. Note: control animals correct the smooth optokinetic tracking with saccadic events, a behavior absent in TeLC animals. e, Average saccadic events per trial per session (Wilcoxon rank sum test, control: 5 animals, 6 sessions, TeLC, 6 animals, 7 sessions, same for c and d). f, Schematic of pupillary reflex paradigm. g, Average stimulus-evoked pupil dilation per animal (control 5 animals, TeLC 7 animals). h, Quantification of the stimulus-evoked dilation speed from g (Wilcoxon rank sum test, same for i and k). i, Median pupil area during still periods for control and TeLC animals. j, Pupil area distribution during running for control and TeLC animals. k, Median pupil area during running periods for control and TeLC animals. OKR, optokinetic reflex.
