Fig. 1: Isolation of single cells from cortical GM, WM and the SEZ.
From: Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter
a, Schematic representation of the workflow used to dissect all three regions from 2–3-month-old C57BL/6J mice to generate the single-cell suspension for scRNA-seq. b, t-distributed stochastic neighbor embedding (t-SNE) visualization of scRNA-seq data from the three regions, with cells color-coded based on their cell types. c, t-SNE visualization showing expression levels (normalized) of common markers for astrocytes. d, Gene expression profiles obtained from scRNA-seq data, segregated according to the cell types identified in b and representing genes used to calculate the astrocyte score. e, Cell type scores used to annotate the scRNA-seq dataset in b. For the complete list of genes used for each cell type, see Supplementary Table 1.
