Extended Data Fig. 7: Characterization of proliferative white matter astrocytes.
From: Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter
a,b, Confocal images of Aldh1l1-eGFP mice immunostained for Rpa2 (a) and Thbs4 (b) after 4 weeks of 5-EdU administration. Dots represent astrocytes in the images (see color code below the image). Scale bars: 100 μm and 50 μm (insets). Quantifications show Rpa2+/GFP+ (a) or Thbs4+/GFP+ (b) astrocytes in a column (top graphs) and proportions in white/gray matter (bottom graphs). Black dots indicate the amount of Rpa2+/5-EdU+/GFP+ (a) Thbs4+/5-EdU+/GFP+ (b) astrocytes. n = 3 animals. Two-sided Student’s t test. Scale bars: 200 μm (overview), 50 μm (inset). c, Confocal images of Sox9 and RFP from mice 14 days after retrovirus (RV) injection into the white matter to label dividing cells. Overview of the ventricular zone/white matter (on the left) and zoom (on the right). Scale bars: 50 μm and 10 μm (insets). d, Confocal images of mice 5 days, 14 days, and 4 weeks after RV injection into the SEZ. Scale bars: 100 μm. e, Confocal images of Aldh1l1-Cre-eGFP white matter stained for Sox9, Gfap, and GFP. Scale bars: 50 μm. Graphs display mean ± s.e.m. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.
